課程名稱 |
超解析顯微技術 Super?resolution microscopy techniques |
開課學期 |
110-2 |
授課對象 |
電機資訊學院 電機工程學研究所 |
授課教師 |
楊東霖 |
課號 |
BEBI5014 |
課程識別碼 |
945 U3030 |
班次 |
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學分 |
3.0 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期二2,3,4(9:10~12:10) |
上課地點 |
明達203 |
備註 |
總人數上限:24人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/1102BEBI5014_ |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
The light microscopy is one of the most widely used tools in biology and physics. Recent advances in super-resolution light microscopy have brought the concept of nanoscale or sub-nanoscale optical resolution into reality. This course will first introduce the fundamental knowledge of optics. We will then cover the fundamentals of light microscopy, modern light microscopy, advanced fluorescence microscopy and super-resolution microscopy. Different super-resolution modalities will be detailed. We will also introduce emerging techniques such as correlative microscopy, label-free imaging, expansion microscopy, etc. |
課程目標 |
- To provide fundamental knowledge of optics, photonics, and light microscopy system.
- To help students develop physical understanding about the underlying principles of various optical and microscopic techniques in modern scientific endeavor.
- To motivate individuals into this exciting research field. |
課程要求 |
- Not specifically required.
- Basic knowledge of optics will be necessary. |
預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
待補 |
參考書目 |
- Bahaa Saleh and Malvin Teich, Fundamental of Photonics, 2nd ed. Wiley, New York, 2007.
- Erfle, Holger, Super-Resolution Microscopy: Methods and Protocols, Humana Press, 2017. |
評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
第1週 |
2/15 |
Introduction |
第2週 |
2/22 |
Fundamentals of optics (I) |
第3週 |
3/01 |
Fundamentals of optics (II) |
第4週 |
3/08 |
Fundamentals of optics (III) |
第5週 |
3/15 |
Light microscopy |
第6週 |
3/22 |
Light microscopy
Fluorescence Microscopy |
第7週 |
3/29 |
Fluorescence Microscopy
Stimulated emission depletion microscopy |
第8週 |
4/05 |
(holiday) |
第9週 |
4/12 |
midterm |
第10週 |
4/19 |
Stimulated emission depletion microscopy |
第11週 |
4/26 |
Localization microscopy |
第12週 |
5/03 |
Localization microscopy |
第13週 |
5/10 |
Structured illumination microscopy |
第14週 |
5/17 |
Multiphoton microscopy |
第15週 |
5/24 |
Emerging techniques |
第16週 |
5/31 |
Oral presentations-term paper |
第17週 |
6/07 |
Proposal Submission Deadline |
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